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KMID : 0878220110230030374
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Journal of Sasang Constitutional Medicine
2011 Volume.23 No. 3 p.374 ~ p.390
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The Effect of Suhwagije-tang Distillate on the Immune Activity of Spleen Cells of Aged Rats
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Choi Jae-Song
Bae Na-Young
Ahn Taek-Won
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Abstract
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Objectives: The purpose of this study is to investigate the effect of Suhwagije-tang(SGT) distillate on the immune activity of spleen cells of aged SD rats.
Methods:We used 10, 50, 72 weeks old SD rats in this study. Spleen cells from SD rats were stimulated with ConA and treated with 1% Vitamin C(Vit.C) or Suhwagijetang distillate(SGT). After 24 hours, the concentrations of IL-2, IL-4, IL-10, IFN- in the cell culture supernatant were measured by ELISA.
Results and Conclusions : 1) At all concentration of SGT distillate, survival rates of liver cells were higher than the control group. In addition, 50% SGT distillate group’s cell survival rates were significantly higher than other groups. 2) In 10 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group, Vit.C group. In 52 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group. 3) In 10, 52 weeks SD rats(SGT group), the concentration of IL-4 significantly decreased in comparison with ConA group. 4) In 10 weeks SD rats(SGT group), the concentration of IL-10 significantly decreased in comparison with ConA group. And in 72 weeks SD rats(SGT group), the concentration of IL-10 significantly increased in comparison with Vit.C group. 5) In 52, 72 weeks SD rats(SGT group), the concentration of IFN- significantly decreased in comparison with 10 weeks SD rats(SGT group). These results suggest that Suhwagije-tang(SGT) distillate has the effect of increasing the immune activity of spleen cells of aged SD rats.
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KEYWORD
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Suhwagije-tang, Immune activity, Spleen cells, Cytokine, Aged rats
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